General Information

  • ID:  hor005033
  • Uniprot ID:  P82965
  • Protein name:  Antidiuretic factor A
  • Gene name:  NA
  • Organism:  Tenebrio molitor (Yellow mealworm beetle)
  • Family:  NA
  • Source:  Animal
  • Expression:  NA
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Tenebrio (genus), Tenebrioninae (subfamily), Tenebrionidae (family), Tenebrionoidea (superfamily), Cucujiformia (infraorder), Polyphaga (suborder), Coleoptera (order), Endopterygota (cohort), Neoptera (infraclass), Pterygota (subclass), Dicondylia, Insecta (class), Hexapoda (subphylum), Pancrustacea, Mandibulata, Arthropoda (phylum), Panarthropoda, Ecdysozoa, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005179 hormone activity; GO:0005184 neuropeptide hormone activity
  • GO BP:  GO:0007218 neuropeptide signaling pathway
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  VVNTPGHAVSYHVY
  • Length:  14(1-14)
  • Propeptide:  VVNTPGHAVSYHVY
  • Signal peptide:  NA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Strong inhibitor of fluid secretion by the Malpighian tubules. Uses cGMP as a second messenger and inhibits fluid production by decreasing cAMP concentration. Reduces isosmotic fluid secretion by inhibiting electroneutral and non-conductive transport path
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: 10 fM
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  NA

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor005033_AF2.pdbhor005033_ESM.pdb

Physical Information

Mass: 177477 Formula: C71H103N19O20
Absent amino acids: CDEFIKLMQRW Common amino acids: V
pI: 7.7 Basic residues: 2
Polar residues: 6 Hydrophobic residues: 5
Hydrophobicity: 18.57 Boman Index: -330
Half-Life: 100 hour Half-Life Yeast: >20 hour
Half-Life E.Coli: >10 hour Aliphatic Index 90
Instability Index: 665 Extinction Coefficient cystines: 2980
Absorbance 280nm: 229.23

Literature

  • PubMed ID:  11756661
  • Title:  Identification of a potent antidiuretic factor acting on beetle Malpighian tubules.
  • PubMed ID:  11893763
  • Title:  Antagonistic control of fluid secretion by the Malpighian tubules of Tenebrio molitor: effects of diuretic and antidiuretic peptides and their second messengers.
  • PubMed ID:  15235016
  • Title:  The mec